Mesenchymal to epithelial transition driven by canine distemper virus infection of canine histiocytic sarcoma cells contributes to a reduced cell motility in vitro.

This study hypothesizes that a CDV infection of canine histiocytic sarcoma cells (DH82 cells) triggers the MET process leading to a decreased cellular motility. Immunofluorescence and immunoblotting were used to investigate the expression of epithelial and mesenchymal markers followed by scratch assay and an invasion assay as functional confirmation. Furthermore, microarray data were analysed for genes associated with the MET process, invasion and angiogenesis. CDV-infected cells exhibited an increased expression of epithelial markers such as E-cadherin and cytokeratin 8 compared to controls, indicating a MET process. This was accompanied by a reduced cell motility and invasiveness. Summarized, these results suggest that CDV infection of DH82 cells triggers the MET process by an increased expression of epithelial markers resulting in a decreased cell motility in vitro.
PMID: 32627957 [PubMed - as supplied by publisher]

Source: J Cell Mol Med - July 5, 2020 Category: Molecular Biology Authors: Armando F, Gambini M, Corradi A, Becker K, Marek K, Pfankuche VM, Mergani AE, Brogden G, de Buhr N, von Köckritz-Blickwede M, Naim HY, Baumgärtner W, Puff C Tags: J Cell Mol Med Source Type: research