Anti-inflammatory canthin-6-one alkaloids from the roots of Thailand Eurycoma longifolia Jack

AbstractTwo new canthin-6-one alkaloids, 4,9-dimethoxy-5-hydroxycanthin-6-one (1) and 9-methoxy-(R/S)-5-(1-hydroxyethyl)-canthin-6-one (2), together with fifteen known ones were isolated from the roots of ThailandEurycoma longifolia Jack. Among the known canthin-6-one alkaloids, compounds9 and16 were isolated from theEurycoma genus for the first time. Meanwhile, the nitric oxide (NO) inhibitory activities of all isolates were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 cells at 50  µM. Moreover, a dose-dependent experiment was conducted for active compounds1,2,4,6,7,10,12–17 at the concentration of 10, 25, and 50  µM, respectively. Consequently, compounds1,4,6,7,12,14,15, as well as17 were found to inhibit NO release from RAW264.7 cells in a dose-dependent manner.Graphic abstractTwo new canthin-6-one alkaloids, 4,9-dimethoxy-5-hydroxycanthin-6-one (1) and 9-methoxy-(R/S)-5-(1-hydroxyethyl)-canthin-6-one (2), together with fifteen known ones were isolated from the roots of Thailand Eurycoma longifolia Jack. Among them, 1, 4, 6, 7, 12, 14, 15, as well as 17 were found to inhibit NO release from RAW264.7 cells in a dose-dependent manner at the concentration of 10, 25, and 50 µM.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research