Molecules, Vol. 25, Pages 3104: Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages

Molecules, Vol. 25, Pages 3104: Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages Molecules doi: 10.3390/molecules25133104 Authors: Nam-Hun Kim Hong-Ki Kim Ji-Hak Lee Seung-Il Jo Hye-Min Won Gyeong-Seok Lee Hyoun-Su Lee Kung-Woo Nam Wan-Jong Kim Man-Deuk Han The NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inflammasome has been implicated in a variety of diseases, including atherosclerosis, neurodegenerative diseases, and infectious diseases. Thus, inhibitors of NLRP3 inflammasome have emerged as promising approaches to treat inflammation-related diseases. The aim of this study was to explore the effects of juglone (5-hydroxyl-1,4-naphthoquinone) on NLRP3 inflammasome activation. The inhibitory effects of juglone on nitric oxide (NO) production were assessed in lipopolysaccharide (LPS)-stimulated J774.1 cells by Griess assay, while its effects on reactive oxygen species (ROS) and NLRP3 ATPase activity were assessed. The expression levels of NLRP3, caspase-1, and pro-inflammatory cytokines (IL-1β, IL-18) and cytotoxicity of juglone in J774.1 cells were also determined. Juglone was non-toxic in J774.1 cells when used at 10 μM (p < 0.01). Juglone treatment inhibited the production of ROS and NO. The levels of NLRP3 and cleaved caspase-1, as well as the secretion of IL-1β and IL-18, were decreased by treatment with juglone in a concentration-dependent manne...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research