MicroRNA expression profile in extracellular vesicles derived from ALV-J infected chicken semen.
This study was to examine the miRNA expression profile in seminal EVs and inquire into the relation between it and the vertical transmission by performing gene ontology (GO) and pathway enrichment analysis. Here, we first isolated and characterized seminal EVs by Nanoparticle Tracking Analysis、Western Blot and Transmission electron microscopy experiments. By deep sequencing of each EVs miRNA library, 9 typical differentially expressed miRNA, including 6 up-regulated and 3 down-regulated, were identified. Gene target prediction, GO annotation and KEGG pathway enrichment analysis showed possible function associated with these miRNAs. Overall, these findings will increase our understanding of the content and composition of miRNA in seminal EVs and provide new insights into the important role of the seminal EVs miRNAs regulation in ALV-J transmission.
PMID: 32621842 [PubMed - as supplied by publisher]
Source: Virus Research - Category: Virology Authors: Chen S, Liao L, Zhao Q, Zhang X, Li H, Lin W, Chen F, Xie Q Tags: Virus Res Source Type: research
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