Functional Characterization and Transcriptional Analysis of clpP of Xanthomonas campestris pv. campestris.

In this study, the Xcc clpP gene which is annotated to encode the proteolytic core of Clp was characterized. Mutation of clpP resulted in susceptibility to high temperature and puromycin stresses. Site-directed mutagenesis revealed that S105, H130, and D179 are critical amino acid residues for ClpP function in puromycin tolerance. Inactivation of clpP also revealed an attenuation of virulence on the host plant and a reduction in the production of extracellular cellulase, mannanase, pectinase, and protease. The affected phenotypes of the clpP mutant could be complemented to wild-type levels by the intact clpP gene. Transcriptional analysis revealed that expression of clpP is induced under heat shock condition.
PMID: 32623486 [PubMed - as supplied by publisher]

Source: Current Microbiology - July 3, 2020 Category: Microbiology Authors: Li CE, Liao CT, Lo HH, Hsiao YM Tags: Curr Microbiol Source Type: research