Rapid Assessment of Cell Wall Polymer Distribution and Surface Topology of Arabidopsis Seedlings.
We describe two rapid assessment techniques that allow for elucidation of the cell wall polymers of root hairs and high-resolution analysis of surface features of various vegetative organs. Live organism immunolabeling of cell wall polymers employing light microscopy and confocal laser scanning microscopy can be effectively performed using a large microplate-based screening strategy (see Figs. 1 and 2). Rapid cryofixation and imaging of variable pressure scanning electron microscopy also allows for imaging of surface features of all portions of the plant as clearly seen in Fig. 3.
PMID: 32617942 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tierney ML, Sun L, Domozych DS Tags: Methods Mol Biol Source Type: research