c-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism.

c-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism. Colloids Surf B Biointerfaces. 2020 Jun 25;194:111211 Authors: Madhyastha H, Madhyastha R, Thakur A, Kentaro S, Dev A, Singh S, Chandrashekharappa R B, Kumar H, Acevedo O, Nakajima Y, Daima HK, Aradhya A, Nagaraj P N, Maruyama M Abstract Green synthesis of metal-encased nutraceutical nano-hybrids has been a target for research over the last few years. In the present investigation, we have reported temperature dependent facile synthesis of silver nanoparticles using FDA approved c phycocyanin (cPC). The cPC conjugated silver nanoparticles (AgcPCNPs) were characterized by TEM, Zeta Potential, UV-vis, XPS, FTIR, and CD Spectroscopy. The temperature optimization studies suggested the synthesis of stable AgcPCNPs at 40 °C while at higher temperature system shows aggregated appearance. Molecular docking studies predicted the exclusive interaction of C, D, I, and J chains of cPC with the surface of AgNPs. Moreover, AgcPCNPs significantly (p < 0.1 %) counteract the toxic nature of AgNPs on red blood cell by measuring parameters like total RBC count, % hemolysis, % hematocrit, coagulation time, pH, electrolyte concentrations and degree of blood cell lipid peroxidation by the anti-oxidation mechanism. Skin fibroblast in vitro cell migration result suggeststhat AgcPCNPs enhanced the degree of cell movement towards the wound area. Data obtaine...
Source: Colloids and Surfaces - Category: Biotechnology Authors: Tags: Colloids Surf B Biointerfaces Source Type: research