Development of Pretreatment Protocols for Determination of Soybean β-Conglycinin in Processed Soybean Foods Using Commercial ELISA Kits.

In this study, samples were pretreated by mixing them with rice flour powder prior to extraction of proteins. Then, we used commercially available ELISA kits for detection of allergens that could be present in any contaminating soybean residue. This enabled accurate and highly reproducible quantitation of β-conglycinin content in several processed soybean foods.
PMID: 32612090 [PubMed - in process]

Source: Journal of Nutritional Science and Vitaminology - July 4, 2020 Category: Nutrition Tags: J Nutr Sci Vitaminol (Tokyo) Source Type: research