Platform to study intracellular polystyrene nanoplastic pollution and clinical outcomes

Schematic illustration of the platform and experimental design with 40  nm polystyrene nanoplastics. Human blastocysts and human induced pluripotent stem cells were exposed for 24 hours to polystyrene nanoitems and used for whole transcriptome sequencing and in depth RNA analysis through Next‐Generation Sequencing technology. In preprocessment, gene IDs were tran slated, scaled, normalized, and used for signaling pathways, analysis, and delivery of the report. AbstractIncreased pollution by plastics has become a serious global environmental problem, but the concerns for human health have been raised after reported presence of microplastics (MPs) and nanoplastics (NPs) in food and beverages. Unfortunately, few studies have investigate the potentially harmful effects of MPs/NPs on early human development and human health. Therefore, we used a new platform to study possible effects of polystyrene NPs (PSNPs) on the transcription profile of preimplantation human embryos and human induced pluripotent stem cells (hiPSCs). Two pluripotency genes,LEFTY1 andLEFTY2, which encode secreted ligands of the transforming growth factor ‐beta, were downregulated, whileCA4 andOCLM, which are related to eye development, were upregulated in both samples. The gene set enrichment analysis showed that the development of atrioventricular heart valves and the dysfunction of cellular components, including extracellular matrix, were significantly affected after exposure of hiPSCs to PSNPs. Final...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Stem Cell Technology: Epigenetics, Genomics, Proteomics, And Metabonomics Source Type: research