Enzyme Sampling: Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry (Small 26/2020)

In article numberhttps://doi.org/10.1002/smll.2020005842000584, Milan Mrksich, Horacio D. Espinosa, and co ‐workers report on a method for non‐destructively sampling enzymes multiple times from small populations of cells, using localized electroporation in microfluidic live cell analysis devices. The enzymes are captured in extraction chambers, where they modify immobilized substrates on a self‐ass embled monolayer, and their activity is quantified using self‐assembled monolayers for matrix‐assisted laser desorption/ionization (SAMDI) mass spectrometry.
Source: Small - Category: Nanotechnology Authors: Tags: Inside Front Cover Source Type: research
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