GSE139101 Engineering Neuronal Organoids with Network Activity and Plasticity

Contributors : Rashi Halder ; Maria P Zafeiriou ; Andre FischerSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHuman brain organoids are promising tools for disease modelling and drug development. For proper neuronal network formation excitatory and inhibitory neurons as well as glia need to develop concurrently. Here we report a novel method for directed differentiation and self-organization of induced pluripotent stem cells in a collagen hydrogel towards a highly interconnected neuronal network in a macroscale tissue format. Bioengineered Neuronal Organoids (BENOs) comprise of interconnected excitatory and inhibitory neurons as well as supportive astrocytes and oligodendrocytes. Giant depolarizing potential (GDP)-like events observed within 20-40 days of culture suggest comprehensive network activity. The switch from excitatory to inhibitory GABA activity, an increase in network burst complexity, and reduced GDPs at>40 day cultures indicated progressive neuronal network maturation. Evidence for paired pulse depression and long-term potentiation suggest neuronal plasticity. BENOs exhibit structural and functional properties and thus may serve as surrogates for the developing human brain.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research