Correlation between intermediary metabolism, Hsp gene expression and oxidative stress related proteins in long-term thermal stressed Mytilus galloprovincialis.

Correlation between intermediary metabolism, Hsp gene expression and oxidative stress related proteins in long-term thermal stressed Mytilus galloprovincialis. Am J Physiol Regul Integr Comp Physiol. 2020 Jul 01;: Authors: Feidantsis K, Giantsis IA, Vratsistas A, Makri S, Pappa AZ, Drosopoulou E, Anestis A, Mavridou E, Exadactylos A, Vafidis D, Michaelidis B Abstract Long-term exposure of Mytilus galloprovincialis to temperatures beyond 26oC triggers mussels' mortality. The present study aimed to integratively illustrate the correlation between intermediary metabolism, Hsp gene expression and oxidative stress related proteins in long-term thermally stressed Mytilus galloprovincialis and whether they are affected by thermal stress' magnitude and duration. We accordingly evaluated the gene expression profiles, in the posterior adductor muscle (PAM) and the mantle, concerning hsp70 and hsp90, and the antioxidant defense indicators Mn-SOD, Cu/Zn-SOD, catalase, glutathione S-transferase, and the metallothioneins mt-10 and mt-20. Moreover, we determined antioxidant enzymes' activities, oxidative stress through lipid peroxidation and activities of intermediary metabolism enzymes. The pattern of changes in relative mRNA expression levels indicate that mussels are able to sense thermal stress even when exposed to 22oC and before mussels' mortality is initiated. Data indicates a close correlation between the magnitude and duration of thermal s...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - Category: Physiology Authors: Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research