Complete Photochemical Regulation of 8-17 DNAzyme Activity using Reversible DNA Photo-cross-linking.

Complete Photochemical Regulation of 8-17 DNAzyme Activity using Reversible DNA Photo-cross-linking.
Chembiochem. 2020 Jun 28;:
Authors: Watanabe Y, Fujimoto K
Abstract
The regulation of DNAzyme activity is an important problem for its in vivo applications. We achieved photochemical regulation of DNAzyme activity using reversible DNA photo-cross-linking of 3-cyanovinylcarbazole ( CNV K). The ODN containing CNV K photo-cross-linked to pyrimidine base in complementary strand with a few seconds of photoirradiation and its photoadduct was photo-split by photoirradiation with another wavelength. The activity of photo-cross-linked DNAzyme having CNV K was completely inhibited (OFF state). In contrast, after 312 nm irradiation, the DNAzyme activity was recovered upon addition of substrate strand (ON state). In addition, the photo-cross-linked DNAzyme is prone to enzymatic digestion by exonuclease. This photochemical OFF to ON switching using reversible DNA photo-cross-linking was regulated at desired timing and position; therefore, it may be possible to use it for in vivo application.
PMID: 32596920 [PubMed - as supplied by publisher]

Source: Chembiochem - June 27, 2020 Category: Biochemistry Authors: Watanabe Y, Fujimoto K Tags: Chembiochem Source Type: research