Acute hypotensive effect of diminazene aceturate in Spontaneously Hypertensive Rats: role of NO and Mas receptor.

Acute hypotensive effect of diminazene aceturate in Spontaneously Hypertensive Rats: role of NO and Mas receptor. Clin Exp Pharmacol Physiol. 2020 Jun 30;: Authors: Sartório CL, Pimentel EB, Dos Santos RL, Rouver WN, Mill JG Abstract Diminazene aceturate (DIZE) has been described as an angiotensin-converting enzyme 2 (ACE2) activator. We aimed to investigate DIZE effects on blood pressure (BP) of spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats. BP was recorded in awaked and unrestrained rats 24 h after femoral artery catheterization. DIZE (15 mg/kg, s.c.) produced a fast BP decrease only in SHR (P<0.01). Pre-treatment with L-NAME (10 mg/kg, i.v.) did not change the hypotensive effect on systolic BP whereas mitigated DIZE effect on diastolic BP (∆ Emax: -31±5 DIZE vs. -15±1 mmHg DIZE+L-NAME, P<0.05). BP changes after DIZE remained unchanged after the treatment of rats with A-779 (50 ug/kg, i.v.), a Mas receptor blocker. Vasodilatation curves to DIZE (10-9 to 10-4 M) in mesenteric arteries confirmed the NO-mediation on DIZE effects in SHR, as L-NAME (300 μM) reduced the vascular sensitivity (∆EC50: -5.12±0.09 CONTROL vs. -4,66±0.08 L-NAME, P<0.05) and the magnitude of DIZE effect (A.U.C.: 357.5±8.2DIZE vs. 424.7±11.6 L-NAME, P<0.001), whereas A-779 (1 μM) enhanced DIZE response (A.U.C.: 357.5±8.2 DIZE vs. 309.8±14.7 A-779, P<0.05). Our findings indicate that DIZE acutely reduces the BP in...
Source: Clinical and Experimental Pharmacology and Physiology - Category: Drugs & Pharmacology Authors: Tags: Clin Exp Pharmacol Physiol Source Type: research