Spinophilin-deficient Mice are Protected from Diet-induced Obesity and Insulin Resistance.

Spinophilin-deficient Mice are Protected from Diet-induced Obesity and Insulin Resistance. Am J Physiol Endocrinol Metab. 2020 Jun 30;: Authors: Zhang Y, Song L, Dong H, Kim DS, Sun Z, Boger H, Wang Q, Wang H Abstract Browning of white adipose tissue (WAT) has been shown to reduce obesity and obesity-related complications suggesting that factors that promote WAT browning may have applications in the development of therapeutic strategies for treating obesity. Here, we show that ablation of spinophilin (SPL), a ubiquitously expressed, multidomain scaffolding protein, increases metabolism and improves energy balance. Male and female SPL knockout (KO) and wild type (WT) littermate controls were fed a Chow diet or a high fat diet (HFD). Body weight, hepatic steatosis, glucose and insulin tolerance, physical activity and expression of browning genes in adipose tissues were measured and compared. Male SPL knockout (KO) mice fed a Chow diet were significantly leaner, had lower body weights, and exhibited better glucose tolerance and insulin sensitivity than wild type (WT) littermate controls. When fed a high fat diet (HFD), spl KO mice were protected from increased body fat, weight gain, hepatic steatosis, hyperinsulinemia, and insulin resistance. Physical activity of spl KO mice was markedly increased compared to WT controls. Furthermore, expression of the brown adipocyte marker, UCP-1, and the mitochondrial activity markers, cd137 and c-id...
Source: American Journal of Physiology. Endocrinology and Metabolism - Category: Physiology Authors: Tags: Am J Physiol Endocrinol Metab Source Type: research