Grading TESI: Crypt and villus formation in tissue-engineered small intestine alters with stem/progenitor cell source.

Grading TESI: Crypt and villus formation in tissue-engineered small intestine alters with stem/progenitor cell source. Am J Physiol Gastrointest Liver Physiol. 2020 Jun 29;: Authors: Gilliam EA, Schlieve CR, Fowler KL, Rea JN, Schall KA, Huang S, Spence JR, Grikscheit TC Abstract The small intestine has a remarkable ability to enhance its absorptive and digestive surface area through the formation of villi, a process known as villification. We sought to learn if developing mouse and human tissue-engineered small intestine (TESI) followed known developmental biology routes to villification such as Shh/Ihh and BMP4/FoxF1 signaling in order to identify targets to enhance the development of TESI. After generating TESI from prenatal and postnatal stem cell sources, we evaluated the effect of cell source derivation on villification with a grading scheme to approximate developmental stage. Chi-square analysis compared the prevalence of TESI grade from each stem cell source. RNAscope probes detected genes known to direct villification and the development of the crypt-villus axis in mouse and human development. These were compared in TESI derived from various pluripotent and progenitor cell donor cell types as well as native human fetal and postnatal tissues. Prenatal and pluripotent cell sources form mature villus and crypt-like structures more frequently than postnatal donor sources and there are alternate routes to villus formation. Human ...
Source: American Journal of Physiology. Gastrointestinal and Liver Physiology - Category: Physiology Authors: Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research