Molecular analysis of cecal and tracheal microbiome of heat-stressed broilers supplemented with prebiotic and probiotic.

Molecular analysis of cecal and tracheal microbiome of heat-stressed broilers supplemented with prebiotic and probiotic. Avian Pathol. 2015 Jan 7;:1-28 Authors: Sohail MU, Hume ME, Byrd JA, Nisbet DJ, Shabbir MZ, Ijaz A, Rehman H Abstract The gastrointestinal tract commensal microbiome is important for host nutrition, health and immunity. Little information is available regarding the role of these commensals at other mucosal surfaces in poultry. Tracheal mucosal surfaces offer sites for first-line health and immunity promotion in broilers, especially under stress-related conditions. The present study is aimed at elucidating effects of feed supplementations with mannan-oligosaccharides (MOS) prebiotic and a probiotic mixture (PM) on cecal and tracheal microbiome of broilers kept under chronic heat-stress (HS; 35 ± 2°C). Day-old chickens were randomly divided into five treatment groups: Thermoneutral control (TN-CONT), HS-CONT, HS-MOS, HS-PM, and HS synbiotic (HS-SYN; fed MOS and PM). Cecal digesta and tracheal swabs were collected at d-42 and subjected to DNA-extraction, followed by PCR-denaturing gradient gel electrophoresis (DGGE) and pyrosequencing. The PCR-DGGE dendrograms revealed significant (49.5%SC) differences between cecal and tracheal microbiome. Tracheal microbiome pyrosequencing revealed 9 phyla, 17 classes, 34 orders, 68 families, and 125 genera, while 11 phyla, 19 classes, 34 orders, 85 families, and 165 genera were i...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research