A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

Reverse genetics is used in many laboratories around the world and enables the creation of tailor-made influenza viruses with a desired genotype or phenotype. However, the process is not flawless, and difficul...

Source: Virology Journal - June 23, 2020 Category: Virology Authors: Sushant Bhat, Dagmara Bialy, Joshua E. Sealy, Jean-Remy Sadeyen, Pengxiang Chang and Munir Iqbal Tags: Methodology Source Type: research