CRISPR –Cas9/CRISPRi tools for cell factory construction in E. coli

AbstractThe innovative CRISPR –Cas based genome editing technology provides some functionality and advantages such as the high efficiency and specificity as well as ease of handling. Both aspects of the CRISPR–Cas9 system including genetic engineering and gene regulation are advantageously applicable to the construction of m icrobial cell factories. As one of the most extensively used cell factories,E. coli has been engineered to produce various high value-added chemical compounds such as pharmaceuticals, biochemicals, and biofuels. Therefore, to improve the production of valuable metabolites, many investigations have been performed by focusing on CRISPR –Cas- based metabolic engineering of this host. In the current review, the biology underlying CRISPR–Cas9 system was briefly explained and then the applications of CRISPR–Cas9/CRISPRi tools were considered for cell factory construction inE. coli.

Source: World Journal of Microbiology and Biotechnology - June 24, 2020 Category: Microbiology Source Type: research