Synthesis, characterization and in vitro antibacterial mechanism study of two Keggin-type polyoxometalates.

Synthesis, characterization and in vitro antibacterial mechanism study of two Keggin-type polyoxometalates. J Inorg Biochem. 2020 Jun 10;210:111131 Authors: Zhao M, Fang Y, Ma L, Zhu X, Jiang L, Li M, Han Q Abstract In this article, two Keggin-type polyoxometalates [Co(L)2]3[PMo12O40] (1) and [Co(L)2]3[PW12O40] (2) (HL = 2-acetylpyrazine thiosemicarbazone) were prepared and fully characterized. The compounds are stable in aqueous solution with different pH values and show superior antibacterial activity against Escherichia coli (E. coli: minimal inhibitory concentration (MIC) = 0.00375, 0.12 μg/mL), Agrobacterium tumefaciens (A. tumefaciens: MIC = 0.06, 0.12 μg/mL), Bacillus subtilis (B. subtilis: MIC = 0.015, 0.06 μg/mL) and especially for Staphylococcus aureus (S. aureus: MIC = 0.00048, 0.015 μg/mL) for 1 and 2, respectively. The time kill studies showed the entire killing of specific bacteria during 4 to 8 h. In addition, the possible antibacterial mechanism of compound 1 was explored systematically. The experimental results proved that cell wall/membrane damage, leakage of protein, inhibition of respiratory chain dehydrogenases activity, enhancement of intracellular reactive oxygen species (ROS) and depletion of glutathione (GSH) were the potential causes of bacteria death. PMID: 32563103 [PubMed - as supplied by publisher]
Source: Journal of Inorganic Biochemistry - Category: Biochemistry Authors: Tags: J Inorg Biochem Source Type: research