Simple transport and cryopreservation of cold-stored mouse embryos.

Simple transport and cryopreservation of cold-stored mouse embryos.
Exp Anim. 2020 Jun 17;:
Authors: Mukunoki A, Takeo T, Nakao S, Tamura K, Horikoshi Y, Nakagata N
Abstract
The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 hours. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice.
PMID: 32554941 [PubMed - as supplied by publisher]

Source: Experimental Animals - June 21, 2020 Category: Research Tags: Exp Anim Source Type: research