An ex vivo multiparametric flow cytometric assay using human whole blood to simultaneously measure cytotoxicity and leishmanicidal activities.

An ex vivo multiparametric flow cytometric assay using human whole blood to simultaneously measure cytotoxicity and leishmanicidal activities. Exp Parasitol. 2020 Jun 17;:107940 Authors: Ribeiro JM, Bandeira CC, de Faria BG, Alves MLR, Vieira FO, Giunchetti RC, Uzonna JE, Teixeira-Carvalho A, Peruhype-Magalhães V, de Souza-Fagundes EM Abstract Therapeutic options for the treatment of leishmaniasis are insufficient and need improvements owing to their low efficiency and high toxicity as well as the emergence of resistant strains. The limited number of new drugs for neglected diseases and lack of innovation in your development are still challenges. In this context, the process of discovery and development of biological assays play a pivotal role for the identification of bioactive compounds. The assays currently used for screening of drugs with cytotoxic activity against Leishmania parasites, include different processes that utilize intact parasite (free or intracellular) or specific enzymes of metabolism as a target cell. These assays allow the screening of large numbers of samples followed by more detailed secondary confirmatory assays to confirm the observed activity and assess their toxicity. In the present study, we described the development of a new functional and more complete assay that enables simultaneous assessment of potential anti-Leishmania compounds through evaluation of internalization of fluorescein-labeled L. brazili...
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research