Determination of levofloxacin, ciprofloxacin, moxifloxacin and gemifloxacin in urine and plasma by HPLC-FLD-DAD using pentafluorophenyl core-shell column: Application to drug monitoring.

In this study, a liquid chromatography method for the determination of levofloxacin, ciprofloxacin, moxifloxacin, and gemifloxacin in human urine and plasma was developed for the first time. Efficiency of 5 different columns in the separation of these fluoroquinolones was compared. Experimental parameters that affect the separation such as percentage of organic solvent, pH, temperature, gradient shape, and detector wavelength were optimized by employing a step-by-step approach. Using a pentafluorophenyl core-shell column (100 mm x 4.6 mm, 2.7 μm), the separation of four analytes was accomplished in less than 7.5 min. The developed method was validated for determination of analytes in both urine and plasma with respect to sensitivity, specificity, linearity (r≥0.9989), recovery (79.46-102.69%), accuracy, precision, and stability (85.79-111.07%). The intra- and inter day accuracies were within 89.55-111.94% with relative standard deviations 0.35-8.05%. The feasibility of method was demonstrated by analyzing urine and plasma samples of patients receiving orally levofloxacin, ciprofloxacin, or moxifloxacin. The developed method is suitable for therapeutic drug monitoring of these fluoroquinolones and can be applied to pharmacokinetic and toxicological studies. PMID: 32557742 [PubMed - as supplied by publisher]
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research