Up-regulation of Matrix Metalloproteinase-2 by Scleral Monocyte-derived Macrophages Contributes to Myopia Development.

Up-regulation of Matrix Metalloproteinase-2 by Scleral Monocyte-derived Macrophages Contributes to Myopia Development. Am J Pathol. 2020 Jun 15;: Authors: Zhao F, Wu H, Reinach PS, Wu Y, Zhai Y, Lei Y, Ma L, Su Y, Chen Y, Li F, Liu X, Srinivasalu N, Qu J, Zhou X Abstract Myopia is a leading cause of visual impairment worldwide. This sight compromising condition is associated with scleral thinning, extracellular matrix (ECM) remodeling and inappropriate optical axial length elongation. Even though macrophages are present in the sclera, their involvement in this condition is unknown. By using a form-deprivation myopia (FDM) mouse model, we found that both the scleral macrophage density and their matrix metallopeptidase-2 (MMP-2) expression levels rose in myopic eyes. Partial scleral macrophage depletion by clodronate (CLOD) shifted the refraction towards hyperopia in both the form deprived and the untreated fellow eyes compared with their respective counterparts in the vehicle-injected control mice. However, this procedure did not alter susceptibility to FDM. FDM development was 59% less in the macrophage-specific Mmp-2 deletion (LysMCreMmp-2fl/fl) mice than in their Cre-negative littermates (Mmp-2fl/fl mice). Moreover, the expression of scleral C-C motif chemokine ligand-2 (CCL2), which is a potent monocyte chemoattractant recruiting monocytes to tissue sites, was increased during myopia progression. However, the increase in the densi...
Source: The American Journal of Pathology - Category: Pathology Authors: Tags: Am J Pathol Source Type: research