Molecules, Vol. 25, Pages 2831: Plasma-Based Proteomics Profiling of Patients with Hyperthyroidism after Antithyroid Treatment

Molecules, Vol. 25, Pages 2831: Plasma-Based Proteomics Profiling of Patients with Hyperthyroidism after Antithyroid Treatment Molecules doi: 10.3390/molecules25122831 Authors: Afshan Masood Hicham Benabdelkamel Aishah A. Ekhzaimy Assim A. Alfadda Thyroid hormones critically modulate body homeostasis and haemostasis by regulating energy and metabolism. Previous studies have focused on individual pathways or proteins that are affected by increases in thyroid hormone levels, while an overall plasma proteomic signature of this increased level is lacking. Herein, an integrated untargeted proteomic approach with network analysis was used to identify changes in circulating proteins in the plasma proteome between hyperthyroid and euthyroid states. Plasma from 10 age-matched subjects at baseline (hyperthyroid) and post treatment with carbimazole (euthyroid) was compared by difference gel electrophoresis (DIGE) and matrix-assisted laser desorption/ionization time of flight (MALDI TOF) mass spectrometry (MS). A total of 20 proteins were identified with significant difference in abundance (analysis of variance (ANOVA) test, p ≤ 0.05; fold-change ≥ 1.5) between the two states (12 increased and 8 decreased in abundance in the hyperthyroid state). Twelve protein spots corresponding to ten unique proteins were significantly more abundant in the hyperthyroid state compared with the euthyroid state. These increased proteins were haptoglobin (HP), hemopexin ...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research