Bioassay-Guided Fractionation, Chemical Compositions and Antibacterial Activity of Extracts from Rhizomes of  Globba schomburgkii Hook.f.

Bioassay-Guided Fractionation, Chemical Compositions and Antibacterial Activity of Extracts from Rhizomes of Globba schomburgkii Hook.f. Chem Biodivers. 2020 Jun 15;: Authors: Suekaew N, Na Pombejra S, Kulsing C, Doungchawee J, Khotavivattana T Abstract Bioassay-guided fractionation was conducted on dichloromethane extract from the rhizomes of Globba schomburgkii Hook.f. which have previously been reported as the part with the highest antibacterial activity. 10 fractions and 20 sub-fractions were obtained and evaluated for their potency against various strains of bacteria. The most active sub-fractions were 8 times more effective against Staphylococcus aureus and Micrococcus luteus than the original crude extract. Moreover, 2 pure compounds namely petasol and (E)-15,16-dinorlabda-8(17),11-dien-13-one were successfully isolated and characterized, for the first time from this plant species. Untargeted compound analysis of all fractions and sub-fractions was performed by gas chromatography hyphenated with mass spectrometry, leading to positive identification of 167 compounds according to comparison with the mass spectrum and retention index database, 137 of which have never been reported for G. schomburgkii. The correlation between antibacterial activity and composition of each fraction suggests that the bioactive compounds could be 4,8-β-epoxy-caryophyllene, methyl isocostate, (E)-labda-8(17),12-diene-15,16-dial, α-kessyl acetate, z...
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research