Vibrios from the Norwegian marine environment: Characterization of associated antibiotic resistance and virulence genes

This study investigated the occurrence ofVibrio spp. in water samples, fish and marine bivalves from the temperate Oceanic and Polar Oceanic areas around Norway. Five species,V. alginolyticus (n = 53),V. metschnikovii (n = 38),V. anguillarum (n = 21),V. antiquarius (n = 2) andV. fujianensis (n = 2) were isolated. We examined antimicrobial susceptibility, hemolytic activity and characterized antimicrobial resistance genes and virulence genes by whole genome sequencing. This study found low prevalence of multidrug resistance, and absence of virulence genes of high global concern. AbstractA total of 116Vibrio isolates comprisingV.  alginolyticus (n = 53),V.  metschnikovii (n = 38),V.  anguillarum (n = 21), V.  antiquarius (n = 2), andV.  fujianensis (n = 2) were obtained from seawater, fish, or bivalve molluscs from temperate Oceanic and Polar Oceanic area around Norway. Antibiotic sensitivity testing revealed resistance or reduced susceptibility to ampicillin (74%), oxolinic acid (33%), imipenem (21%), aztreonam (19%), and tobramycin (17%). Wh ole‐genome sequence analysis of eighteen drug‐resistant isolates revealed the presence of genes like β‐lactamases, chloramphenicol‐acetyltransferases, and genes conferring tetracycline and quinolone resistance. The strains also carried virulence genes likehlyA,tlh,rtxA to D andaceA, E and F. The genes for cholerae toxin (ctx), thermostable direct hemolysin (tdh), or zonula occludens toxin (zot) were not detected in...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research