Subcellular localization of Sur7 and its pleiotropic effect on cell wall integrity, multiple stress responses, and virulence of Beauveria bassiana.

Subcellular localization of Sur7 and its pleiotropic effect on cell wall integrity, multiple stress responses, and virulence of Beauveria bassiana. Appl Microbiol Biotechnol. 2020 Jun 16;: Authors: Zhang LB, Tang L, Guan Y, Feng MG Abstract Sur7 is one of multiple proteins constituting MCC (membrane compartment of Can1 acting as an arginine/H+ symporter), a crucial membrane domain that can form punctuate eisosome spots on the plasma membrane and execute diverse functions in model yeast but remains poorly understood in filamentous fungi. Here, a Sur7 homolog bearing a typical SUR7 domain and four transmembrane domains was shown to localize in the conidial vesicles and enter vacuoles and appear sporadically on the periphery membrane during hyphal growth in the insect-pathogenic fungus Beauveria bassiana, implicating an involvement of Sur7 in cellular events linked to both plasma membrane and vacuoles. Deletion of sur7 resulted in reduced conidiation capacity and impaired conidial quality, which was featured by slower germination, attenuated virulence, and reduced carbohydrate epitopes (β-N-acetylglucosamine and sialic acids). Also, the hyphal cell walls of the deletion mutant were severely impaired due to ~ 70% reductions in chitin and neutral carbohydrate contents and a moderate increase in alkali-soluble carbohydrate content. Consequently, the deletion mutant became more sensitive to three cell wall perturbing chemicals (Congo red...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research