14. Characterizing response to checkpoint blockade treatment in mouse urothelial carcinoma model with scRNA

While checkpoint blockade treatment has been shown to be highly effective in various tumor types, effectiveness for urothelial carcinoma has been minimal. To examine how urothelial carcinoma responds to combined PD-L1 and CTLA-4 inhibition, we used single cell RNA sequencing along with whole genome, exome, and bulk RNA sequencing derived from a mouse model with three condition groups: control, checkpoint treatment (Atezolizumab and Ipilimumab), and checkpoint treatment with CD4 depletion. The cell line used for analysis is an organoid-based line developed by inducing invasive urothelial carcinoma in mice through exposure to N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) in drinking water.

Source: Cancer Genetics and Cytogenetics - May 31, 2020 Category: Genetics & Stem Cells Authors: Sharon Freshour, Bryan Fisk, Matthew Mosior, Jennifer Bolzenius, Obi Griffith, Vivek Arora, Malachi Griffith Source Type: research