253 RNA-seq profiling of tape strips from infants with atopic dermatitis show profound barrier and immune abnormalities

Atopic dermatitis (AD) onset usually occurs in children younger than 5 years old. While whole-skin profiling has been the gold standard for identifying AD-related biomarkers in adults, skin biopsies are not feasible in infants. An alternate minimally invasive technique to detect barrier and immune biomarkers in infants with AD is needed. We used RNA-seq to profile 20,000+ transcripts in lesional and non-lesional skin of 19 infants (0-5 y/o) with moderate-to-severe AD and 17 healthy controls. We achieved 100% detection rate of all samples, with 1,890 differentially expressed genes in lesional or non-lesional AD versus controls, using threshold of fold change>2 and false discovery rate (FDR)
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Epidermal Structure and Barrier Function Source Type: research