607 Targeting 14-3-3e-CDC25A interactions to trigger apoptotic cell death in skin cancer

Non-melanoma skin cancer is the most common form of cancer worldwide. We previously documented an anti-apoptotic role for CDC25A in cutaneous squamous cell carcinoma (SCC), an activity dependent on its association with 14-3-3 proteins. We hypothesized that targeting CDC25A-14-3-3 ε interactions may be an effective strategy for inducing skin cancer cell apoptosis. Co-immunoprecipitation revealed that CDC25A associated with 14-3-3ε, 14-3-3γ and 14-3-3ζ in SCC cells but not normal keratinocytes. Additionally, in SCC cells, overexpression of CDC25A induced Akt/BAD/Survivin p ro-survival signaling while knockdown of 14-3-3ε inhibited pro-survival signaling, suggesting that 14-3-3ε and CDC25A similarly activate pro-surivval signaling pathways to suppress cell death.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Pharmacology and Drug Development Source Type: research