Genetically encoded protein labeling and crosslinking in living Pseudomonas aeruginosa.

We report the genetic code expansion of this opportunistic pathogen by using the pyrrolysyl-tRNA synthetase-tRNA system, which enabled the genetic and site-specific incorporation of unnatural amino acids bearing bioorthogonal handles or photo-affinity groups into proteins in PA. This strategy allowed us to conduct bioorthogonal labeling and imaging of flagella, as well as site-specific photo-affinity capturing of interactions between a Type III secretion effector and its chaperone inside living bacteria.
PMID: 32503693 [PubMed - as supplied by publisher]

Source: Bioorganic and Medicinal Chemistry - June 8, 2020 Category: Chemistry Authors: Zheng H, Lin S, Chen PR Tags: Bioorg Med Chem Source Type: research