Selenoprotein I is essential for murine embryogenesis.

Selenoprotein I is essential for murine embryogenesis.
Arch Biochem Biophys. 2020 Jun 02;:108444
Authors: Avery J, Yamazaki Y, Hoffmann FW, Folgelgren B, Hoffmann PR
Abstract
Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI-/+ mating pairs were used to generate global KO offspring. Of 323 weanling pups, no homozygous KO genotypes were found. E6.5-E18.5 embryos (165 total) were genotyped, and only two E18.5 KO embryos were detected with no discernable anatomical defects. To screen embryos prior to uterine implantation that occurs ∼ E6, blastocyst embryos (E3.5-E4.4) were flushed from uteruses of pregnant females and analyzed for morphology and genotype. KO embryos were detected in 5 of 6 pregnant females, and 7 of the 32 genotyped embryos were found to be SELENOI KO that exhibited no overt pathological features. Overall, these results demonstrate that, except for rare cases (2/490 = 0.4%), global SELENOI deletion leads to early embryonic lethality.
PMID: 32502470 [PubMed - as supplied by publisher]

Source: Archives of Biochemistry and Biophysics - June 1, 2020 Category: Biochemistry Authors: Avery J, Yamazaki Y, Hoffmann FW, Folgelgren B, Hoffmann PR Tags: Arch Biochem Biophys Source Type: research