Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity.

Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity. Biomed Microdevices. 2020 Jun 03;22(2):41 Authors: Välimäki H, Hyvärinen T, Leivo J, Iftikhar H, Pekkanen-Mattila M, Rajan DK, Verho J, Kreutzer J, Ryynänen T, Pirhonen J, Aalto-Setälä K, Kallio P, Narkilahti S, Lekkala J Abstract Luminescence-based oxygen sensing is a widely used tool in cell culture applications. In a typical configuration, the luminescent oxygen indicators are embedded in a solid, oxygen-permeable matrix in contact with the culture medium. However, in sensitive cell cultures even minimal leaching of the potentially cytotoxic indicators can become an issue. One way to prevent the leaching is to immobilize the indicators covalently into the supporting matrix. In this paper, we report on a method where platinum(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorphenyl)-porphyrin (PtTFPP) oxygen indicators are covalently immobilized into a polymer matrix consisting of polystyrene and poly(pentafluorostyrene). We study how the covalent immobilization influences the sensing material's cytotoxicity to human induced pluripotent stem cell-derived (hiPSC-derived) neurons and cardiomyocytes (CMs) through 7-13 days culturing experiments and various viability analyses. Furthermore, we study the effect of the covalent immobilization on the indicator leaching and the oxygen sensing properties of the material. In addition, we demonstrate the use of th...
Source: Biomedical Microdevices - Category: Biomedical Engineering Authors: Tags: Biomed Microdevices Source Type: research