Crystal structure of the SH3 domain of growth factor receptor-bound protein 2

This study presents the crystal structure of the N-terminal SH3 (SH3N) domain of growth factor receptor-bound protein 2 (Grb2) at 2.5   Å resolution. Grb2 is a small (215-amino-acid) adaptor protein that is widely expressed and involved in signal transduction/cell communication. The crystal structure of full-length Grb2 has previously been reported (PDB entry 1gri). The structure of the isolated SH3N domain is consistent with the full-length structure. The structure of the isolated SH3N domain was solved at a higher resolution (2.5   Å compared with 3.1   Å for the previously deposited structure) and made it possible to resolve some of the loops that were missing in the full-length structure. In addition, interactions between the carboxy-terminal region of the SH3N domain and the Sos1-binding sites were observed in the structure of the isolated domain. Analysis of these interactions provided new information about the ligand-binding properties of the SH3N domain of Grb2.

Source: Acta Crystallographica Section F - June 4, 2020 Category: Biochemistry Authors: Bolgov, A. Korban, S. Luzik, D. Zhemkov, V. Kim, M. Rogacheva, O. Bezprozvanny, I. Tags: SH3 domain Grb2 adaptor protein growth factor receptor-bound protein 2 research communications Source Type: research