Molecules, Vol. 25, Pages 2622: Generation of High Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat αs1-Casein

Molecules, Vol. 25, Pages 2622: Generation of High Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat αs1-Casein Molecules doi: 10.3390/molecules25112622 Authors: Aliah Zannierah Mohsin Rashidah Sukor Jinap Selamat Anis Shobirin Meor Hussin Intan Hakimah Ismail Nuzul Noorahya Jambari Farina Mustaffa-Kamal The chemical, technological and allergy properties of goat’s milk are significantly affected by the level of αs1-casein. Detection and quantification of αs1-casein requires high-specificity methods to overcome high-sequence similarity between this protein and others in the casein family. Unavailability of antibodies with high affinity and specificity towards goat αs1-casein hinders the development of immuno-based analytical methods such as enzyme-linked immunosorbent assay (ELISA) and biosensors. Here, we report the generation of polyclonal antibodies (or immunoglobulins, IgGs) raised towards goat αs1-casein N- (Nter) and C-terminal (Cter) peptide sequences. The Nter and Cter peptides of goat αs1-casein were immunized in rabbits for the generation of antisera, which were purified using protein G affinity chromatography. The binding affinity of the antisera and purified IgGs were tested and compared using indirect ELISA, where peptide-BSA conjugates and goat αs1-casein were used as the coating antigens. The Nter antiserum displayed higher titer than...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research