Effect of post-prandial lipemia on canine biochemical parameters

This study assessed postprandial lipemia of healthy dogs fed with commercial feed, as well as the effect of lipemia induced in vitro by the addition of commercial lipid solution. Twenty healthy dogs were selected, for induction of postprandial lipemia, and the peak lipemic time (3 h after feeding) was compared with two non-lipemic times (12 h of fasting), 1 day before and 1 day after the induction of lipemia, respectively. Lipid solution was added to non-lipemic sera simulating triglyceride concentrations similar to the minimum, medium, and maximum values obtained at the lipemic time in vivo. Biochemical analyses were performed in semi-automated spectrophotometer using a set of commercial reagents. Variables were tested for normality and differences between the groups were tested using pairedt test or Wilcoxon and ANOVA or Friedman tests. Both in vivo and in vitro lipemia caused significant biochemical changes in routine tests in healthy dogs, varying according to the analyte tested. Results from lipemic samples should be interpreted with caution for some determinations, whereas in some analyses, the need for fasting is dispensable.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research