Response to Comment on "RNA-guided DNA insertion with CRISPR-associated transposases"

Rice et al. suggest that the CRISPR-associated transposase ShCAST system could lead to additional insertion products beyond simple integration of the donor. We clarify the outcomes of ShCAST-mediated insertions in Escherichia coli, which consist of both simple insertions and integration of the donor plasmid. This latter outcome can be avoided by use of a 5' nicked DNA donor.

Source: ScienceNOW - June 3, 2020 Category: Science Authors: Strecker, J., Ladha, A., Makarova, K. S., Koonin, E. V., Zhang, F. Tags: Molecular Biology t-comment Source Type: news