Establishment of Recombinase Polymerase Amplification assay for rapid and sensitive detection of Orientia tsutsugamushi in Southeast Asia.

Establishment of Recombinase Polymerase Amplification assay for rapid and sensitive detection of Orientia tsutsugamushi in Southeast Asia. Acta Trop. 2020 May 31;:105541 Authors: Nguyen CT, Nguyen UD, Le TT, Bui HT, Nguyen ANTTÁ, NguyenAnh ANTTN, Trieu NT, Trieu LP, Bui ST, Nguyen C, Van Hoang L, Ho SA, Van Nguyen B, Stenman J, Ho TH Abstract Scrub typhus, caused by Orientia tsutsugamushi, is a common fever in parts of Southern and South East Asia. As delayed diagnosis of scrub typhus leads to inappropriate treatment and high mortality rates, of up to 70%, sensitive and rapid detection of O. tsutsugamushi is required for timely and appropriate treatment. Molecular assays, such as PCR and real-time PCR, have been shown to be more sensitive than conventional immunoassay, however, they are only available in centralized laboratories. In contrast to PCR assays, Recombinase Polymerase Amplification (RPA) is conducted under a constant temperature ranging from 24°C to 45°C. Therefore, this technology is very promising for nucleic acid testing in the field, and in resource-limited areas. An RPA assay for the detection of O. tsutsugamushi based on the target gene encoding for the 47 kDa outer membrane protein has been reported, but the primer and probe sequences of this assay are suboptimal for detection of the majority of recently published sequences of O. tsutsugamushi isolates from Southeast Asia. We have established a real-time RPA assa...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research