A Semi-Automated Method for Regional Cerebral Uptake Analysis in Micro PET-CT

Conclusions: A method has been developed that uses freely available software to co-register a mouse brain atlas to a cohort of micro PET-CT images, enabling uptake assessment of cerebral VOIs with minimal computational demand. References: 1Janke AL, Ullmann JF. (2015) Robust methods to create ex vivo minimum deformation atlases for brain mapping. Methods. 2015 Feb;73:18-26. doi: 10.1016/j.ymeth.2015.01.005 2M. Jenkinson, C.F. Beckmann, T.E. Behrens, M.W. Woolrich, S.M. Smith. FSL. NeuroImage, 62:782-90, 2012 Figure 1: (Top row) A skull-cropped CT image in the Sagittal and Transaxial planes with the skull cavity mask shown in yellow. (Bottom row) The same CT overlaid with the co-registered AMBMC template; Olfactory bulb and Cerebellum VOIs shown in green and blue respectively. Figure 2: Sagittal and Transaxial views of the co-registered binary skull-cropped CT masks showing the voxel-wise registration accuracy from 0 to 100%. Figure 3: The Olfactory bulb/Cerebellum VOI uptake ratios for the wild type control mouse and the averaged Alzheimer’s Amyloidosis APP/PS1 transgenic mice.
Source: Journal of Nuclear Medicine - Category: Nuclear Medicine Authors: Tags: Basic Science - New tracers/analysis Source Type: research