Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams

This study aimed to determine the phylogenetic relationships between gum ‐producingLeuconostoc andLactobacillus strains isolated from various locations in a sugarcane processing factory at times when sugars with a low ‐ and high‐dextran content were produced. AbstractHigh levels of gums such as dextran, produced byLeuconostoc andLactobacillus spp., have a severe impact on factory throughput and sugar quality. This study aimed to determine the phylogenetic relationships between gum ‐producingLeuconostoc andLactobacillus bacteria which were isolated from various locations in a sugarcane processing factory at times when low ‐ and high‐dextran raw sugar, respectively, were produced. Phylogenetic analysis of 16S rRNA gene sequences grouped 81 isolates with the type strains ofLeuconostoc mesenteroides (subspp.mesenteroides,dextranicum, andcremoris),Leuconostoc pseudomesenteroides, Leuconostoc lactis, andLeuconostoc citreum, respectively. Forty ‐three isolates clustered with the type strain ofLactobacillus fermentum. The phylogenetic relatedness of the isolates was determined by sequencing and analysis of the housekeeping genesrpoA anddnaA forLeuconostoc spp. and thepheS andtuf genes for theLactobacillus spp. TherpoA gene proved discriminatory for the phylogenetic resolution of all of the isolatedLeuconostoc spp. and thednaA housekeeping gene was shown to be effective for isolates clustering with the type strains ofLeuc.  mesenteroides andLeuc.  citreum. None of the ...

Source: MicrobiologyOpen - June 3, 2020 Category: Microbiology Authors: Sanet Nel, Stephen B. Davis, Akihito Endo, Leon M. T. Dicks Tags: ORIGINAL ARTICLE Source Type: research