Molecular characteristics of dengue viruses in patients hospitalized at the Bamrasnaradura Infectious Diseases Institute, Thailand.

The objective of the present study, conducted in 2018 at the Bamrasnaradura Infectious Diseases Institute, was to characterize the DENV genotype distribution among severe dengue patients. A total of 100 hospitalized severe dengue patients were enrolled with written informed consent. Serum specimens were tested by multiplex real-time reverse transcription-polymerase chain reaction. A total of 94 cases were DENV detected, with 46 DENV-1, 38 DENV-2, 10 DENV-4, and no DENV-3 cases. Sequence determination of the DENV envelope-protein gene was successful in 73 cases. Genotyping of the resultant sequences revealed 40 DENV-1 genotype I, 26 DENV-2 including 18 genotype Cosmopolitan and 8 genotype Asian I, and 7 DENV-4 genotype I cases. DENV-1 was the most prevalent in the current study, and the ratio of DENV-2 genotype Cosmopolitan apparently increased since our previous study. These results suggest that genotypic diversity might be increasing in Thailand. (200 words). PMID: 32475871 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research