An ultra-rapid real-time RT-PCR method for detecting human orthopneumovirus using PCR1100.

In this study, an ultra-rapid real-time RT-PCR method for the rapid detection of RSV was developed using the PCR1100 device based on the US CDC assay in order to detect RSV in comparable time to rapid test kits. The ultra-rapid real-time RT-PCR could detect RSV viral RNA in less than 20 min while maintaining sensitivity and specificity comparable to conventional real-time RT-PCR using large installed instruments. Furthermore, combining ultra-rapid real-time RT-PCR with the M1 Sample Prep kit reduced the total working time for the detection of RSV from clinical specimen to less than 25 min, suggesting this method could be used for point-of-care RSV testing. PMID: 32475879 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/32475879?dopt=Abstract

Source: Japanese Journal of Infectious Diseases - May 28, 2020 Category: Infectious Diseases Authors: Shirato K, Nao N, Kawase M, Kageyama T Tags: Jpn J Infect Dis Source Type: research