Optimizing a real-time PCR assay for rapid detection of Candida auris in nasal and axillary/groin samples.

Conclusion . The optimized procedure was used to test 1414 patient surveillance samples. The real-time PCR detected all culture-positive samples with 100 % sensitivity and 100 % specificity. PMID: 32478655 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research

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AbstractPurpose of reviewThere is a continuing debate regarding contact precaution (CP) usage for endemic multidrug-resistant organisms (MDROs). In this review, we examine current recommendations for CP and highlight differences in CP use between endemic and non-endemic MDROs.Recent findingsThe discontinuation of CP had no effect on the incidence of methicillin-resistantStaphylococcus aureus and vancomycin-resistantEnterococci. The evidence regarding CP for extended-spectrum beta-lactamase producing Enterobacteriaceae is inconclusive, highlighting the need for more research to determine best infection control strategies. C...
Source: Current Treatment Options in Infectious Diseases - Category: Infectious Diseases Source Type: research
Publication date: Available online 14 June 2020Source: Parasitology InternationalAuthor(s): Mateus Damiani Monteiro, Eurípedes Gomes de Carvalho Neto, Iuri Pereira dos Santos, Marcela Santiago Biernat, Rodrigo Mazeron Machado, Vitor Bonetti Hauser, Carlos R.M. Rieder, Candida Nubia Spengler, Vanessa Pascoal Fey, Alessandra Morassutti, Vitor Félix Torres, Raphael Machado Castilhos, Carlos Graeff-Teixeira
Source: Parasitology International - Category: Parasitology Source Type: research
The recent emergence of a multidrug-resistant yeast, Candida auris, has drawn attention to the closely related species from the Candida haemulonii complex that include C. haemulonii, Candida duobushaemulonii, Candida pseudohaemulonii, and the recently identified Candida vulturna. Here, we used antifungal susceptibility testing and whole-genome sequencing (WGS) to investigate drug resistance and genetic diversity among isolates of C. haemulonii complex from different geographic areas in order to assess population structure and the extent of clonality among strains. Although most isolates of all four species were genetically...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Abstract Candida auris has emerged as a multi-drug resistant nosocomial pathogen over the last decade. Outbreaks of the organism in healthcare facilities has resulted in life-threatening invasive candidiasis in over 40 countries worldwide. Resistance by C. auris to conventional antifungal drugs such as fluconazole and amphotericin B means that alternative therapeutics must be explored. As such, this study served to investigate the efficacy of a naturally derived polysaccharide called chitosan against aggregative (Agg) and non-aggregative (non-Agg) isolates of C. auris in vitro and in vivo In vitro results indicate...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
AbstractCandida auris is a remarkable emerging pathogen. It has emerged separately, yet simultaneously in different parts of the world, establishing four phylogenetic and geographic distinct clades with a potential fifth clade that was recently reported.C. auris is often perceived as a pathogen in disguise, due to its frequent misidentification and its immune evasion. On the other hand, many of the recovered isolates are multidrug-resistant. In fact, some of these isolates are resistant to the three main antifungal classes: echinocandins, azoles, and polyenes. Moreover,C. auris has the ability to persist and survive on dif...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
Candida auris is a recently emerged multidrug-resistant fungal pathogen causing severe illness in hospitalized patients. C. auris is most closely related to a few environmental or rarely observed but cosmopolitan Candida species. However, C. auris is unique in the concern it is generating among public health agencies for its rapid emergence, difficulty to treat, and the likelihood for further and more extensive outbreaks and spread. To date, five geographically distributed and genetically divergent lineages have been identified, none of which includes isolates that were collected prior to 1996. Indeed, C. auris’ ecol...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Candida auris is a multidrug-resistant yeast which has emerged in health care facilities worldwide; however, little is known about identification methods, patient colonization, environmental survival, spread, and drug resistance. Colonization on both biotic (patients) and abiotic (health care objects) surfaces, along with travel, appear to be the major factors for the spread of this pathogen across the globe. In this investigation, we present laboratory findings from an ongoing C. auris outbreak in New York (NY) from August 2016 through 2018. A total of 540 clinical isolates, 11,035 patient surveillance specimens, and 3,67...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Mycology Source Type: research
Candida auris is an emerging human fungal pathogen that is being increasingly linked to outbreaks. It is concerning to health care workers because of its high mortality rate, due primarily to its antifungal resistance. Among the tools being developed to study this yeast are large cohorts of regional isolates, which can be useful for studying epidemiology, antifungal susceptibility patterns, and diagnostic methods. In this issue of the Journal of Clinical Microbiology, Y. Zhu, B. O’Brien, L. Leach, A. Clarke, et al. (J Clin Microbiol 58:e01503-19, 2020, https://doi.org/10.1128/JCM.01503-19) describe the laboratory fin...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Commentary Source Type: research
Several case reports and cohort studies have examined the use of (1,3)-beta-d-glucan measurement with cerebrospinal fluid to diagnose fungal meningitis. This systematic review aims to characterize the evidence regarding cerebrospinal fluid (1,3)-beta-d-glucan measurement to detect fungal meningitis. We searched PubMed for (1,3)-beta-d-glucan and each of several distinct fungi, cerebrospinal fluid, and meningitis. Summary data including diagnostic performance (where applicable) were recorded. A total of 939 records were examined via a PubMed search. One hundred eighteen records remained after duplicates were removed, and 10...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Mycology Source Type: research
AbstractPurpose of reviewWe review T2Candida panel performance in diagnosing invasive candidiasis, potential roles of T2Candida, and other culture-independent tests in patient management and antifungal stewardship, and diagnosis and treatment of infections byCandida auris, which has emerged globally as a cause of nosocomial outbreaks.Recent findingsIn 2 multicenter trials, T2Candida was 90% sensitive and 98% specific for diagnosing candidemia directly from whole blood samples. A subsequent study demonstrated T2Candida sensitivity/specificity of 45%/96% for diagnosing deep-seated candidiasis. Other studies have shown that o...
Source: Current Treatment Options in Infectious Diseases - Category: Infectious Diseases Source Type: research
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