Role of Platelet C ‐Type Lectin‐Like Receptor 2 in Promoting Lung Metastasis in Osteosarcoma

ABSTRACTThe overall prognosis of patients with sarcoma ‐based cancers has changed little in the last 20 years. There is an urgent need to investigate the metastatic potential of these tumors and to develop anti‐metastatic drugs. It is becoming increasingly clear that platelets play an important role in the establishment of metastasis of carcinoma cells and could be a useful therapeutic target for patients with carcinoma. However, little is known about the role of platelets in sarcoma progression. Here, we investigated how osteosarcoma progression relates to platelet function to explore the possibility of anti‐platelet therapy. We found tha t, similar to carcinoma cells, podoplanin (also known as Aggrus)‐positive osteosarcoma cells induce platelet aggregation and activation. Administration of anti–glycoprotein Ibα (GPIbα, also known as CD42b) antibody reduced the lung metastasis of osteosarcoma. The supernatant from platelets coc ultured with osteosarcoma cells contained several growth factors and promoted proliferation, invasiveness, and sphere formation of osteosarcoma cells in vitro. In addition, the development of lung metastasis was highly dependent on direct interaction between osteosarcoma cells and platelets. To expl ore the therapeutic target, we focused on the interactions between podoplanin on osteosarcoma and C‐type lectin‐like receptor (CLEC)‐2 on platelets. The administration of a depleting antibody against CLEC‐2 efficiently suppressed oste...
Source: Journal of Bone and Mineral Research - Category: Orthopaedics Authors: Tags: Original Article Source Type: research