GSE146240 Polysomes bypass a 50 nucleotide coding gap less efficiently than monosomes due to attenuation of an unstable 5 ’ mRNA stem loop stimulator and enhanced drop-off

Contributors : Sin éad O’Loughlin ; Mark C Capece ; Mariia Klimova ; Norma M Wills ; Arthur Coakley ; Ekaterina Samatova ; Patrick O’Connor ; Gary Loughran ; Jonathan S Weissman ; Pavel V Baranov ; Marina V Rodnina ; Joseph D Puglisi ; John F AtkinsSeries Type : OtherOrganism : Escherichia coli ; Escherichia virus T4Ribosome profiling analysis of phage T4 infected E. coli yielded protected mRNA fragments within the normal size range derived from ribosomes stalled at the take-off codon. Ribosomes at this position also yielded some 53 nucleotide fragments, 16 longer. These were due to protection of the nucleotides that form the 5 ’ stem loop.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Other Escherichia coli Escherichia virus T4 Source Type: research

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