Investigation of the effects of squalene and squalene epoxides on the homeostasis of coenzyme Q10 in rats by UPLC-Orbitrap MS.

Investigation of the effects of squalene and squalene epoxides on the homeostasis of coenzyme Q10 in rats by UPLC-Orbitrap MS. Chem Biodivers. 2020 May 29;: Authors: Yang H, Yu W, Sun K, Zhang L, Chen C, Su R, Liu S, Wang H, Liu Y Abstract Squalene has been used as a dietary supplement for a long history due to its potential cancer-preventive function. However, the mechanism has not yet been investigated in detail. Therefore, the aim of this study is to see if the plasma coenzyme Q 10 (CoQ 10 ) level will be altered by gavage of squalene and oxidosqualenes to rats. In the present work, a sensitive and simple high-performance analytical method based on ultra-high-performance liquid chromatography coupled with an Orbitrap mass spectrometry (UPLC-Orbitrap-MS) was developed for the quantification of CoQ 10 in rat plasma. Coenzyme Q 9 (CoQ 9 ) was employed as the internal standard. CoQ 10 was determined after acetonitrile-mediated plasma protein precipitation using UPLC-Orbitrap-MS in negative ion mode. Intragastric administration of squalene and the two squalene epoxides into rats once daily for several days elevated the level of CoQ 10 in their plasma, but there was no significant difference between high-dose ( 286 mg/kg ) and low-dose ( 143 mg/kg ) groups. Intragastric administration of squalene once a day for 5 consecutive days and oxidosqualens once a day for 3 consecutive days is necessary for reaching the steady-state level of CoQ ...
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research