Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti ‑PD‑1 administration.

Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration. Mol Med Rep. 2020 May 22;: Authors: Zhang L, Wang IM, Solban N, Cristescu R, Zeng G, Long B Abstract The MC38 (derived from carcinogen‑induced colon adenocarcinoma) tumor model is sensitive to anti‑programmed cell death‑1 (anti-PD‑1) treatment. However, there is no comprehensive description of the T and B cell receptor (TCR, BCR) repertoires of the MC38 tumor model following anti‑PD‑1 treatment, an improved understanding of which is highly important in the development of anti‑PD‑1 immunotherapy. The present study analyzed the TCR and BCR repertoires of three types of tissue, including tumor, spleen and tumor draining lymph node (DLN) from 20 MC38 syngeneic mice receiving murine anti‑PD‑1 (mDX400) treatment or mouse immunoglobulin G1 (mIgG1) control treatment. To obtain enough tissues for high‑throughput sequencing, samples were collected on day 8 after the start of initial treatment. The usage frequencies of seven TCR β chain (TRB) V genes and one TRBJ gene were significantly different between mDX400‑ and mIgG1‑group tumors. TCR repertoire diversity was significantly lower in mDX400‑group tumors compared with mIgG1‑group tumors, with the top 10 most frequent TCR clonotypes notably expanded in mDX400‑group tumors. In addition, the proportion of high‑frequency ...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research