Diverse Secondary Metabolites from a Lichen-Derived Amycolatopsis Strain.

In this study, the secondary metabolites of a lichen-derived actinomycete strain Amycolatopsis sp. YIM 130687 were investigated intensively by using three different media (4#, 302#, and 312#) for fermentation. A total of 21 compounds were isolated from the fermented extraction of the strain. The structures of all compounds were identified by the examination of HRESIMS and NMR spectra. Compounds 1-3, 5, 6, 21 were only found in the cultivation on 302# medium, while compounds 4, 9-11 were only obtained when the strain was cultured on 312# medium. On the other hand, compounds 7, 8, and 20 were only isolated from the fermentation product on 4# medium. The antimicrobial activity test showed that compound 9 had significant inhibitory effects on bacterial pathogens of Staphylococcus aureus and MRSA with the MICs of 2 μg/ml and fungal pathogens of Botrytis cinerea and Fusarium graminearum with the MICs of 1 μg/ml. PMID: 32468182 [PubMed - as supplied by publisher]
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research

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Conclusion: The pediatric population had a high frequency of burn injuries, representing an incentive for more focused educational prevention programs in that group. Additionally, burn infections carry significant morbidity, and are associated with longer hospital stay. These data can help implement various prevention programs. PMID: 32601641 [PubMed - in process]
Source: Saudi Medical Journal - Category: Middle East Health Tags: Saudi Med J Source Type: research
This study aimed to examine...
Source: BMC Microbiology - Category: Microbiology Authors: Tags: Research article Source Type: research
This study included 25 DAP-S/DAP-NS isogenic, clinically-derived MRSA bloodstream isolates. MICs were performed to DAP; nafcillin (NAF; PBP-promiscuous), cloxacillin (LOX; PBP-1), ceftriaxone (CRO; PBP-2) and cefoxitin (FOX; PBP-4). Three DAP-NS isolates were selected for 28-day serial passage in subinhibitory β-lactams. DAP MICs and time-kill assays, host defense peptide (LL-37) susceptibilities and whole genome sequencing were performed to associate genetic changes to key phenotypic profiles.Pronounced decreases in baseline MICs were observed for NAF and LOX (but not for CRO or FOX) among DAP-NS vs DAP-S isolates (&...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
CONCLUSION: This study demonstrated a high frequency of corneal MDRO infections and specifically MRSA and Pseudomonas spp. isolates. Antibiotic treatments frequently required adjustment. Further prospective study of visual outcomes from ophthalmic MDRO cultures is needed. PMID: 32588895 [PubMed - as supplied by publisher]
Source: Military Medicine - Category: International Medicine & Public Health Tags: Mil Med Source Type: research
Vancomycin is among last-resort drugs for the elimination of serious methicillin-resistant Staphylococcus aureus (MRSA) infections. Suboptimal or prolonged exposure to vancomycin is a major cause of decreased vancomycin susceptibility being a great concern toward the eradication of related infections. This arises from genetic and metabolic alterations leading to cell wall thickness and mitigation of autolysis. Therefore, the study on the major mechanisms contributing to the development of heterogeneous vancomycin-intermediate S. aureus (hVISA) and VISA strains and development of novel and efficient therapeutic approaches i...
Source: Reviews in Medical Microbiology - Category: Microbiology Tags: BACTERIOLOGY Source Type: research
This study evaluated the chemical compounds present in Eucalyptus citriodora essential oil (EOEc) cultivated in northeastern Brazil and its properties as an antibacterial agent and resistance modifier against methicillin-resistant Staphylococcus aureus (MRSA) and β-lactamase-producing strains. The EOEc was obtained using the hydrodistillation method, later analyzed by GC/MS, presenting a total of twelve compounds, with citronellal (65.45%); citronellol (14.87%); isopulegol (11.80%) and citronellyl acetate (2.51%) as its main constituents. The microdilution test was used to determine the minimum inhibitory concentratio...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research
Literature regarding the value of pre-operative nasal methicillin resistant Staphylococcus aureus (MRSA) swabs to predict surgical site infections (SSIs) in children undergoing lower extremity surgery is limited. The purpose of our study was to determine if pre-operative nasal MRSA swab results were predictive of SSI development in children undergoing a femoral varus derotational osteotomy (VDRO). Patients who underwent VDRO between 2004–2016 were reviewed to determine pre-operative MRSA colonization rates and SSI devolvement rates. Patients with less than 1 year of follow-up, previous history of infections, or abse...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
We present a case of severe CAP caused by PVL-positive MRSA with ensuing septic shock. Patient concerns: A 68-year-old male with no concerning medical history had developed a fever that reached 39.0°C, a productive cough that was sustained for 5 days, and hypodynamia. He was treated with azithromycin and alexipyretic in a nearby clinic for 2 days in which the symptoms were alleviated. However, 1 day later, the symptoms worsened, and he was taken to a local Chinese medicine hospital for traditional medicine treatment. However, his clinical condition deteriorated rapidly, and he then developed dyspnea and hemoptysis. ...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Clinical Case Report Source Type: research
Pneumonia is the leading cause of morbidity and mortality in the United States. The American Thoracic Society and The Infectious Diseases Society of America recently published updated guidelines for the diagnosis and treatment of community-acquired pneumonia. Initial antibiotic therapy should cover for Streptococcus pneumoniae, Haemophilus influenzae, and other gram-negative bacilli. Patients who have risk factors for the development of antibiotic resistant pathogens, such as methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa should have appropriate empiric coverage for these pathogens. The recommended ...
Source: Infectious Diseases in Clinical Practice - Category: Infectious Diseases Tags: Clinical Guidelines Source Type: research
Authors: Phuyal N, Jha PK, Raturi PP, Rajbhandary S Abstract Crude methanol extracts of fruits, seeds, and bark of Zanthoxylum armatum were investigated in vitro for antimicrobial activities against 9 different bacterial strains: Bacillus subtilis, Enterococcus faecalis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus epidermidis using agar well diffusion method, and the MBC values were determined. Only 5 bacteria, i.e., Bacillus subtilis, Enterococcus faecalis, MRSA, Staphylococcus aureus...
Source: Journal of Tropical Medicine - Category: Tropical Medicine Tags: J Trop Med Source Type: research
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