Genetic mapping of quantitative trait loci and a major locus for resistance to grey leaf spot in maize.

Genetic mapping of quantitative trait loci and a major locus for resistance to grey leaf spot in maize. Theor Appl Genet. 2020 May 28;: Authors: Du L, Yu F, Zhang H, Wang B, Ma K, Yu C, Xin W, Huang X, Liu Y, Liu K Abstract KEY MESSAGE: The genetic basis of GLS resistance was dissected using two DH populations sharing a common resistant parent. A major QTL repeatedly detected in multiple developmental stages and environments was fine mapped in a backcross population. Grey leaf spot (GLS), caused by Cercospora zeae-maydis or Cercospora zeina, is a highly destructive foliar disease worldwide. However, the mechanism of resistance against GLS is not well understood. To study the inheritance of this resistance, we developed two doubled haploid (DH) populations sharing a common resistant parent. The two DH populations were grown in two locations representing the typical maize-growing mountain area in Southwest China for 2 years. GLS disease severity was investigated 2 or 3 times until maturity in the 2 years, and the area under the disease progress curve was calculated. Two high-density linkage maps were constructed by genotyping-by-sequencing. A total of 22 quantitative trait loci (QTLs) were detected for GLS resistance, with most QTLs being repeatedly detected in different stages, locations and years. The confidence intervals of two major QTLs (qGLS_Y2-2 and qGLS_Z2-1) on chromosome 2 from the two DH populations overlapped with each ot...
Source: TAG. Theoretical and Applied Genetics - Category: Genetics & Stem Cells Authors: Tags: Theor Appl Genet Source Type: research